|
KMID : 0380620090410030258
|
|
Korean Journal of Food Science and Technology
2009 Volume.41 No. 3 p.258 ~ p.264
|
|
|
Estimation of Measurement Uncertainty for the HPLC Analysis ofDeoxynivalenol in Wheat
|
|
Ok Hyun-Ee
Chang Hyun-Joo
Ahn Jang-Hyuk
Cho Jae-Young
Chun Hyang-Sook
|
|
|
Abstract
|
|
|
|
The principal objective of this study was to estimate the measurement uncertainty associated with determinationof deoxynivalenol (DON), a mycotoxin generated by Fusarium strain, in food. In service of this goal, wheat as a foodmatrix was analyzed via high performance liquid chromatography-ultraviolet (HPLC-UV) detection using an immunoaffinitycolumn for clean-up. The uncertainty sources in the measurement process were identified by sample weight, final volume,and sample concentration in extraction volume with components including standard stock solution, working standardsolution, 5 standard solutions, calibration curve, matrix, and instrument. The expanded uncertainty for DON at aconcentration of 300μg/kg was estimated as 71.62μg/kg using a coverage factor of two, which provides a confidencelevel of approximately 95%. The most influential component in the uncertainty sources was the recovery of the wheatmatrix, followed by the calibration curve. These results indicate that all efforts may be directed toward reducing theuncertainties of the recovery of the wheat matrix and the calibration curve to obtain a reliable HPLC-UV method for DONanalysis in wheat.
|
|
|
KEYWORD
|
|
|
deoxynivalenol, wheat, HPLC, uncertainty, immunoaffinity column
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|